Fig. 4: Use of the cell map in driving studies of subcellular structure and function.
From: Multimodal cell maps as a foundation for structural and functional genomics
a, The results from AlphaFold-Multimer folding of heterodimeric protein complexes in the U2OS cell map. b,c, SEC–MS plot (b) and the corresponding structure (c) for the DPYSL2–DYSL3 heterodimer. d,e, SEC–MS plot (d) and the structure (e) for TARS3 and EPRS1. f,g, SEC–MS plot (f) and the structure (g) for ERH and CCDC9B, excluding disordered regions. h, IF images for representative members of the Rag–Ragulator complex. Members are immunostained (green) with cytoskeleton counterstain (red). Scale bar, 2 µm. i, Biophysical interaction data for the Rag–Ragulator complex. j, Integrative structure model of the Rag–Ragulator complex. The structural ensembles of ITPA and BORCS6 are presented as 3D localization probability densities, with surfaces transparent for visual clarity. k, Biophysical interaction data for representative RNase MRP complex members. l, IF images for four RNase MRP proteins, immunostained (green) and with cytoskeleton counterstain (red). Scale bar, 5 µm. m, Differential expression (z score, colour bar) after CRISPR knockdown of genes encoding the RNase MRP complex (top rows, green) versus a random sampling of other proteins. The rows represent CRISPR knockdowns, and the columns represent genes with the 20 most variable differential expression patterns across the full dataset.
