Extended Data Fig. 1: Data quality assessment.
From: Multimodal cell maps as a foundation for structural and functional genomics
a) Complete network of U2OS protein-protein interactions as measured by AP-MS. b) Histogram of number of interactions per protein. c) Fraction of CORUM complexes significantly enriched (1% FDR, Methods) for AP-MS interactions measured in USOS (left) alongside interactions ascertained in two previously published AP-MS networks for other cell lines22,51 (middle and right). Error bars denote 95% confidence intervals. d) Interaction networks for select CORUM complexes, with FDR q-values as per panel (c). Blue nodes denote bait proteins and grey nodes denote prey proteins. e) PANTHER106 classifications of protein function (top 30 largest classes by number of proteins), shown for proteins covered by U2OS cell map in comparison to the entire human proteome (UniProt, downloaded September 11, 2023). f) Protein pairs ranked by cosine similarity in AP-MS features enrich for the most similar protein pairs (top 1% in the immunofluorescent protein image features and g) vice versa.
