Fig. 3: Increased activity in PV+ interneurons leads to upregulation of Scg2 and Vgf.
From: Regulation of PV interneuron plasticity by neuropeptide-encoding genes
a, Experimental strategy. Adapted from ref. 68 (reprinted with permission from AAAS) and ref. 69. b, Volcano plot showing ribosome-associated mRNAs identified through RNA-seq. Differentially expressed RNAs (fold change > 1.5, P < 0.05, two-sided Wald’s test with adjustment for multiple testing) are labelled in teal. c, Ranking of the top DEGs on the basis of four selection criteria (Methods). Darker shades indicate higher scores (values 0 to 1). d, Partial STRING network of the most prominent node of DEGs, highlighting IEGs, and genes encoding secreted and membrane-bound proteins. e, Scg2 and Vgf expression in neighbouring hM3Dq+ and uninfected PV+ interneurons in layer 2/3 of S1 from CNO-treated mice. Scale bar, 10 µm. f, Expression of Scg2 (n = 6 mice; two-tailed paired Student’s t-test, P = 0.01) and Vgf (n = 5 mice; two-tailed paired Student’s t-test, P = 0.01) in neighbouring hM3Dq+ and uninfected PV+ interneurons from CNO-treated mice. Data are mean ± s.e.m. CoV, coefficient of variation.
