Extended Data Fig. 1: hM3Dq infection density and CNO control.
From: Regulation of PV interneuron plasticity by neuropeptide-encoding genes
a, mCherry and PV expression in coronal sections through S1. b, quantification of the percentage of mCherry+ PV+ interneurons in layer 2/3 (n = 6 mice). c, Experimental strategy. Adapted from ref. 68 (reprinted with permission from AAAS) and ref. 69. d, Traces of mEPSCs recorded from mCherry-infected PV+ interneurons in vehicle- and CNO-treated mice. e, Quantification of amplitude (vehicle, n = 9 cells, 9 slices, 3 mice; CNO, n = 10 cells, 6 slices, 3 mice; two-tailed Student’s t-test, p = 0.51) and frequency (vehicle, n = 9 cells, 9 slices, 3 mice; CNO, n = 10 cells, 6 slices, 3 mice; two-tailed Student’s t-test, p = 0.45) of mEPSCs recorded from mCherry-infected PV+ interneurons in vehicle- and CNO-treated mice. f, Traces of mIPSCs recorded from mCherry-infected PV+ interneurons in vehicle- and CNO-treated mice. g, Quantification of amplitude (vehicle, n = 9 cells, 9 slices, 3 mice; CNO, n = 10 cells, 6 slices, 3 mice; two-tailed Student’s t-test, p = 0.64) and frequency (vehicle, n = 9 cells, 9 slices, 3 mice; CNO, n = 10 cells, 6 slices, 3 mice; two-tailed Student’s t-test, p = 0.84) of mIPSCs recorded from mCherry-infected PV+ interneurons in vehicle- and CNO-treated mice. h, Excitation/Inhibition (E/I) ratio in mCherry-infected PV+ interneurons in vehicle- and CNO-treated mice (vehicle, n = 9 cells; CNO, n = 10 cells; two-tailed Student’s t-test, p = 0.24). Data are presented as mean ± s.e.m. Scale bar, 50 µm.
