Extended Data Fig. 9: Pathways for complex pollutant degradation by VCOD-15.
From: Bioremediation of complex organic pollutants by engineered Vibrio natriegens
a-e, Catabolic pathways and LC-MS spectra for degradation intermediates of biphenyl (1) (a), phenol (6) (b), naphthalene (8) (c), dibenzofuran (15) (d), and toluene (18) (e). The organic pollutants were added to resting cell suspensions in nine-salt solution; metabolites were extracted by ethyl acetate after six hours (see Methods and Materials for details). Detected biphenyl degradation intermediates included biphenyl-2,3-diol (3), 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (4), and benzoic acid (5). Catechol was a detected degradation intermediate from phenol (7). Detected naphthalene degradation intermediates included naphthalene-1,2-diol (10) and salicylic acid (14). Detected dibenzofuran degradation intermediates included 2,2’,3-trihydroxybiphenyl (16) and salicylic acid (14). Detected toluene degradation intermediates included benzyl alcohol (19) and benzoic acid (5).
