Extended Data Fig. 10: Fento-1 induces ferroptosis in CD44high iron-rich cancer cells (Part 3).
From: Activation of lysosomal iron triggers ferroptosis in cancer
a, ICP-MS of blood, serum and lymphatic fluid from mice. Blood and serum: n = 7 mice; Lymph: n = 6 mice. 1-way ANOVA. b, HMRhoNox-M flow cytometry of dissociated mouse tumour cancer cells. n = 10 mice. Each coloured dot represents a tumour of a distinct mouse. Two-sided Mann–Whitney test. c, Tumour diameter in mice treated with Fento-1 (0.003 mg per animal every-other-day). 2-way ANOVA. Data are mean ± s.e.m. 2nd experiment: vehicle n = 10 mice, Fento-1 n = 5 mice; 3rd experiment: n = 10 mice for each group; 4th experiment: n = 5 mice for each group. d, Tumour diameter in tumour-bearing mice treated with Fento-1 (0.003 mg compound per animal every-other-day). n = 4 independent experiments. 1st experiment: n = 5 mice for each group; 2nd experiment vehicle: n = 10 mice, Fento-1: n = 5 mice; 3rd experiment: n = 10 mice for each group; 4th experiment: n = 5 mice for each group. e, Mice body weight of mice treated as in Fig. 3h and Extended Data Fig. 10c. 1st experiment: n = 5 mice for each group; 2nd experiment: vehicle n = 10 mice, Fento-1 n = 5 mice; 3rd experiment: n = 10 mice for each group; 4th experiment: n = 5 mice for each group. Data are mean ± s.e.m. f, Lipidomics of lysophospholipids in tumours of tumour-bearing mice treated with Fento-1 as in Fig. 3j. n = 4 4T1 tumours. LPC lipids displayed. LPE, LPS, Supplementary Information. Box plots: interquartile range, centre lines = medians and whiskers = the minimum and maximum values.
