Extended Data Fig. 5: Global synapse and glia number are unaffected by Hs-HARE5.
From: A human-specific enhancer fine-tunes radial glia potency and corticogenesis
a, Representative images of adult brain sections stained for SYN1 (green) and DRAQ5 (red), Mask used to exclude cells from synapse quantifications (black). b-c, Quantification of SYN1+ puncta in layer II/III (b) and layer V (c) of S1 in adult control (n = 3), HARE5Hs/Mm and HARE5Hs/Hs (n = 5) brains from 3 litters. Values are calculated as fraction of non-DRAQ5 labeled pixels. d-g, Glia staining on P21 control and HARE5Hs/Hs brains. Representative images of OLIG2 (grey) (d) and SOX9 (grey)(e). f-g, Quantification of global cell number of oligodendrocytes (f) and astrocytes (g) in P21 control (n = 4) and HARE5Hs/Hs (n = 3) brains from 3 litters. Scale bars: 50 µm (a),100 µm (d, e). All values represent the mean ± s.d., Statistics on animals; colors (f,g), littermates. Student’s unpaired, two-tailed t-test (b, c, f, g). Images of sections were captured in entirety using tiling, which creates appearance of some artificial lines.
