Extended Data Fig. 7: scRNA-seq indicates cell composition of Hs-HARE5 cortices.
From: A human-specific enhancer fine-tunes radial glia potency and corticogenesis
a, Experimental paradigm for scRNA sequencing of E14.5 control (n = 2) and HARE5Hs/Hs (n = 2) cortices from 2 litters. b-e, UMAP of RGCs (b) and IPs (d) from all 4 samples. Quantification of subcluster proportions of RGCs (c) and IPs (e). *P = 0.02 (RGC-9); *P = 0.04 (RGC-11). *P = 0.01 (IP-0,1,9); *P = 0.02 (IP-6). Cell clusters were defined by gene lists from Supplementary Table 1. f, Gene Ontology analysis of molecular function showing statistically Differentially Expressed Genes (DEGs) in HARE5Hs/Hs RGCs. The x-axis indicates the fold change in expression. DEGs are listed in Supplementary Table 1. P values were calculated based on a two-tailed Fisher test (c, e).
