Extended Data Fig. 8: Hs-HARE5 promotes progenitor cell cycle exit at mid-stages of cortical development.

a, Cartoon shows an overview of BrdU pulse chase from E13.5 to E14.5. b, Representative sections of E14.5 control and HARE5^Hs/Hs brains stained for BrdU (green), KI67 (red) and DAPI (blue). c, Quantification of cell cycle exit in E14.5 control (n = 4) and HARE5^Hs/Hs (n = 4) cortices from 2 litters. d, Cartoon shows an overview of BrdU pulse chase from E14.5 to E15.5. Below is an overview of cell types labeled and generated from this pulse-chase experiment. e, Representative sections of E15.5 control and HARE5^Hs/Hs brains stained for BrdU (green), KI67 (red), TBR2 (grey) and DAPI (blue). f, Quantification of cell cycle exit of TBR2+ cells in E15.5 control (n = 4) and HARE5^Hs/Hs (n = 5) cortices from 3 litters. BrdU+TBR2 + KI67- cells reflect a subset of TBR2+ cells which have exited the cell cycle. g, Representative sections of E15.5 control and HARE5^Hs/Hs brains stained for BrdU (green), KI67 (red), SOX2 (grey) and DAPI (blue). High magnification view of progenitors, right. h, Quantification of cell cycle exit of SOX2+ cells in E15.5 control (n = 4) and HARE5^Hs/Hs (n = 5) cortices from 3 litters. **P = 0.0086. BrdU+SOX2 + KI67- cells reflect a subset of SOX2+ cells which have exited the cell cycle. For data in (f,h), this is an underestimate of all exiting progenitors due to the labels used and timing of BrdU pulse relative to the cell cycle of progenitors. Scale bars: 50 µm (b, e, g), 20 µm (g-inset). All values represent the mean ± s.d. Statistics on animals; colors, littermates. P values were calculated based on the student’s unpaired, two-tailed t-test (c, f, h).

Source data