Extended Data Fig. 4: Correlation analysis of resting state activity of each individual mouse.
From: A human-specific enhancer fine-tunes radial glia potency and corticogenesis
a, Spatial maps show the pixelwise correlation value threshold of independence across the imaging field of view for each mouse. b-d, A similar analysis as Fig. 1 with (b) maps showing the temporal correlation between each pixel and the average time course of the functional cluster to which it belongs; and (c) correlation maps were spatially binned into a grid of 10×10 pixels and compared between control(n = 3) and HARE5Hs/Hs and HARE5Hs/Mm (n = 4) brains from 2 litters. P-value map shows statistically significant spatial grids as calculated using two-tailed t-test (All non-black pixels are statistically significant, p < 0.05). (d) Functional clusters were grouped into known anatomical regions based on the Allen Atlas Common Coordinate Framework. Secondary motor cortex (M2) was split rostral-caudally and evaluated separately. Graphs show average Pearson’s correlation (r) within regions rM2; cM2; S1bf; RSP, between control (n = 3) and HARE5Hs/Hs and HARE5Hs/Mm (n = 4) from 2 litters. **P = 0.0078 (S1bf); **P = 0.0058 (cM2); **P = 0.0097 (RSP). e, Spatial maps show pixelwise correlation of each pixel to its own functional cluster (within region correlation) for each individual mouse. All values represent the mean ± s.d. Statistics on animals (d). Student’s unpaired, two-tailed t-test (d).
