Extended Data Fig. 4: PAMmla feature importance for enzymes targeting different PAM classes. | Nature

Extended Data Fig. 4: PAMmla feature importance for enzymes targeting different PAM classes.

From: Custom CRISPR–Cas9 PAM variants via scalable engineering and machine learning

Extended Data Fig. 4: PAMmla feature importance for enzymes targeting different PAM classes.The alternative text for this image may have been generated using AI.

SHapely Additive exPlanations (SHAP)48 analysis to investigate the impact of amino acid substitutions (i.e. PAMmla features) on model output for each of the 16 NGNN PAMs. SHAP values are shown for 200 enzymes sampled from the training set. Top 10 features with highest mean absolute SHAP values (greatest absolute impact on model output) are plotted for each PAM.

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