Extended Data Fig. 10: Global analysis of cellular architectures of clones.

a) Summary of approach to randomizing cell types across clones and iteratively generating these clones. Created in BioRender; Nowakowski, T. (2024) https://BioRender.com/moeg7qu. b) Number of unique cell types found per individual clone plotted against the size of the individual clone. Point size represents the number of clones with a given size and number of unique cell types. c) Number of unique cell types per clone after shuffling cell types found in each clone. d) Number of clones identified with a given number of unique cell types. Clones were randomly generated 1,000 times. Gray points indicate the mean number of clones from the 1,000 iterations and error bars represent the 95% CI. P-values represent the Bonferroni-corrected significance for a two-sided permutation test. e) Composition of clones that contain either an oRG or tRG. Randomized distributions were generated by first restricting to clones that include either an oRG or tRG, then shuffling cell types found in each clone 1,000 times. Graph shows all clone types that were found at least twice in either oRG- or tRG-containing clones in the observed data. f-g) Number of clones for each two-cell clone type for e) ≤GW20 or f) >GW20 samples, comparing mean and 95% CI of a randomized distribution vs. observed counts. Randomized distributions were generated by first restricting to ≤GW20 or >GW20 samples, then shuffling cell types found in each clone 1,000 times. Cell types were broadly reclassified as progenitor (P: RG, EX_IPC, IN_IPC), neuron (N: EX, INs), or glia (G: OPC, Oligodendrocyte, Astrocyte).