Extended Data Fig. 7: TCRs in the brain are clonally expanded and highly overlap with certain tissues.
From: The subfornical organ is a nucleus for gut-derived T cells that regulate behaviour
a, Percentage of unique T cell clones per tissue and line graph representing raw number of unique TCR clones in each tissue from the mouse single-cell TCR-seq dataset. b, Demultiplexed brain sample according to unique Hashtag antibody barcode (TotalSeq-C Hashtag 1–6) labelling each mouse. c, TCR clonal sharing as measured by the Morisita index (n = 5). d, FACS analysis of CD4 T cells in Smarta TCR (Vα2Vβ8) transgenic mouse. *P < 0.01 by unpaired t-test (n = 4 per group, pooled 2 experiments in white fat and mLN; repeated once). e, FACS analysis of Smarta Rag1–/– mice that were immunized using LPS as an adjuvant and co-administered with vehicle or GP61–80 peptide by oral route to prime antigens through the gut. (n = 4 per group) f, Same as in e, with an additional boost of vehicle, LPS or LPS and GP61–80 peptide (n = 3 per group). g, 50,000 naive 7B8 T cells were transferred into gnotobiotic mice administered GF or SFB faecal slurry. FACS analysis of the absolute number and frequency of TH17 7B8 T cells from white fat, mLN, small intestine and spleen 5 weeks later. *P < 0.05, **P < 0.01 by unpaired parametric t-test (n = 5 per group; repeated twice). h, Same as in g, except 50,000 naive 7B8 T cells were transferred into Jackson Laboratory (no SFB) or Taconic Biosciences (have SFB) mice. *P < 0.05 by unpaired parametric t-test *P < 0.05 by unpaired parametric t-test (n = 4 per group).
