Extended Data Fig. 10: Behavioural tests in additional transgenic mice deficient in brain T cells or IFNGR.
From: The subfornical organ is a nucleus for gut-derived T cells that regulate behaviour
a, Smarta Rag KO or heterozygous (het) mice were subjected to the NSFT, b, LDT, c, OFT, d, TST and two days of FST. (Pooled 3 experiments for males and females). e, Cxcr6 KO mice were subjected to the NSFT, f, LDT, g, OFT, h, TST and FST. (Pooled 3 experiments for males and females). i, Ifngr1f/fCx3cr1-cre mice were subjected to the LDT, j, OFT, k, TST and FST. (Pooled 4 experiments for males and females) l, Bulk RNA-seq of FACS sorted microglia from wild-type and Tbet KO mice. Separate PCA plot by sex. m, Volcano plot of DEGs. DEGs (cut-off FDR < 0.05) were used for QIAGEN IPA. n, Ifngr1f/fNestin-cre mice were subjected to the LDT, o, OFT, p, TST and FST. q, Ifngr1f/fGfap-cre mice were subjected to the LDT, r, OFT, s, TST and FST. t, Single-cell suspensions were isolated from the brains of Ifng KO mice and prepared for FACS to identify microglia and astrocytes. (n = 3 per group; pooled 2 experiments for females) Statistical tests: *P < 0.05, **P < 0.01 and ***P < 0.001 by two-way ANOVA with Bonferroni’s multiple comparisons test for a–k,n–t. Outliers were calculated using the ROUT outlier test (Q = 1%) and excluded. Images in l were created in BioRender.
