Extended Data Fig. 3: Mouse brain T cells secrete various cytokines and express co-inhibitory receptors.
From: The subfornical organ is a nucleus for gut-derived T cells that regulate behaviour
a, Single-cell suspensions isolated from brain, meninges, white fat and spleen were stimulated with PMA–ionomycin and incubated with Brefeldin A at 37 °C for four hours. Representative flow-cytometric plots of IFNγ and IL-17A double producing cells present in the brain at steady-state. b, Representative flow-cytometric expression of IL-17A from brain, meninges, white fat and spleen. Single-cell suspensions isolated from brain, meninges, white fat, colon, brown fat, liver, lung, ovaries, spleen, and lymph nodes (mesenteric (m)LN, inguinal (i)LN and deep cervical (dc)LN) were stimulated with PMA–ionomycin and incubated with GolgiPlug at 37 °C for four hours. ****P < 0.0001 one-way ANOVA with Tukey’s multiple comparison’s test (n = 4 per group pooled from 3 experiments). c, Representative flow-cytometric expression of transcription factor FOXP3 and cytokine IL-10 from steady-state brain, meninges, white fat and spleen. d, Representative flow-cytometric expression of transcription factor GATA3 and cytokine IL-4 staining in the steady-state brain, spleen and lung. e, Representative flow-cytometric expression of co-inhibitory receptors PD-1, TIGIT and LAG3 in the steady-state brain, spleen and lung.
