Fig. 4: CSF tracer in lymphatics around the eye and in the nasal sidewall, hard palate and superficial cervical regions of the monkey.
From: Increased CSF drainage by non-invasive manipulation of cervical lymphatics
a, Sequence of intracisternal infusion of 2.5 ml indocyanine green (ICG) into M. fascicularis monkeys over 10 min followed at 30 min by imaging of ICG in the face and neck. Before infusion, 1.0 ml of CSF was removed at the cisterna magna over 10 min. b–e, Images of the face of monkeys before and after the intracisternal infusion showing ICG fluorescence (white) in lymphatics. The white asterisk (bone–cartilage junction) marks the lymphatic connection between the nasal mucosa and nasal sidewall. The green arrows indicate ICG in lymphatics in the periorbital and nasal sidewall regions and in scLVs. The yellow arrows indicate ICG in the smLN. Representatives of n = 3 monkeys from three independent experiments. f, Image showing ICG fluorescence (white) in hard palate and buccal lymphatics and scLVs (green arrowheads) of a monkey after intracisternal infusion. The white dashed line marks the boundary of the hard palate. Representatives of n = 3 monkeys from three independent experiments. Anatomical positions are indicated in the bottom right corner.
