Fig. 7: Increased CSF drainage in aged mice by mechanical stimulation of scLVs.
From: Increased CSF drainage by non-invasive manipulation of cervical lymphatics
a, Sequence of intracisternal infusion of 1.0 μl TMR–dextran over 1 min into aged Prox1–GFP mice (87–105 weeks of age) followed 10 min later by sham or low-magnitude mechanical stimulation of intact skin over 20 min beginning 10 min after TMR–dextran infusion. TMR–dextran fluorescence was imaged and measured in the ipsilateral smLN at 30 min. b,c, Fluorescence images (b) and measurements (c) of TMR–dextran fluorescence in the smLN after ipsilateral low-magnitude mechanical stimulation of the skin or no stimulation (sham) over 20 min. Scale bars, 500 μm. Each dot is the TMR–dextran fluorescence intensity of the smLN in one mouse. n = 6 mice per group from three independent experiments. The error bars indicate mean ± s.e.m. P values were calculated by two-tailed Mann–Whitney U-test. d, Sequence of intracisternal infusion of 1.0 μl TMR–dextran over 1 min into aged Prox1–GFP mice (87–105 weeks of age) followed by sham or low-magnitude mechanical stimulation of intact skin over 5 min beginning 30 min after TMR–dextran infusion. TMR–dextran fluorescence was imaged and measured in ipsilateral scLV-1 and scLV-2 at 35 min. e,f, Fluorescence images and measurements of TMR–dextran fluorescence in scLV-1 and scLV-2 at 35 min after ipsilateral low-magnitude mechanical stimulation of the skin or no stimulation (sham) over 5 min. Anatomical positions are shown in the bottom left corner. Scale bars, 200 μm. Each dot is the value of the combined TMR–dextran fluorescence intensity of scLV-1 and scLV-2 from one mouse. n = 5 (sham) and n = 4 (low-magnitude mechanical stimulation) mice per group from three independent experiments. The error bars indicate mean ± s.e.m. P values were calculated by two-tailed Mann–Whitney U-test.
