Extended Data Fig. 6: Anatomical output of SNr to latRM, MRN and SC.
From: Dynamic basal ganglia output signals license and suppress forelimb movements
a, (left) Schematic representing viral injection scheme of a fluorescent protein (Tag) in the SNr to visualize projections to the brainstem (n = 3, Allen Connectivity map data). (centre) Mean Tag density across mice in CCF levels encompassing the lateral medulla. Sections are spaced 200 micrometres. Top is dorsal and right is lateral. 7 N: Facial nucleus. (right) Mean Tag density across mice in CCF levels encompassing the midbrain reticular nucleus (MRN) and superior colliculus (SC). Sections are spaced 100 micrometres. Top is dorsal and right is lateral. Yellow asterisk: SNr. b, (left) Schematic representing viral injection scheme of a Cre-dependent synaptophysin fused fluorescent protein tag (SynTag) in the SNr to visualize collaterals of SNr>latRM projection neurons expressing Cre through retrograde targeting via G-deleted Rabies (n = 3). (centre) Mean Tag density across mice in CCF levels encompassing the lateral medulla. Sections are spaced 200 micrometres. Top is dorsal and right is lateral. 7 N: Facial nucleus. (right) Mean Tag density across mice in CCF levels encompassing the MRN and SC. Sections are spaced 100 micrometres. Top is dorsal and right is lateral. Yellow asterisk: SNr. Note that caudal lateral SNr projects to latRM, MRN and SC and SNr>latRM projection neurons collateralize in the MRN and lateral SC.
