Fig. 1: Multimodal genomic taxonomy of cell types in the human PFC and cell-type-specific gene expression changes.
From: Single-cell transcriptomic and chromatin dynamics of the human brain in PTSD
a, Schematic of the analyses. TF, transcription factor. b, Uniform manifold approximation and projection (UMAP) of snRNA-seq (n = 935,371 nuclei) across 14 subtypes (top) and cell proportion of subtypes across diagnostic conditions (CON, MDD and PTSD; bottom). *FDR < 0.05. c, UMAP of snATAC-seq (n = 473,033 nuclei) across seven cell types (top) and proportion of cell types across conditions (bottom). d, UMAP of snMultiome (n = 119,431 nuclei) across 14 subtypes (top) and proportions across conditions (bottom). e, Significant DEG counts in both directions for each major cell type (left), EXN subtypes (right top) and IN subtypes (right bottom) coloured by the number of DEGs. f, Binary plot indicating occurrence of n = 1,184 PTSD snDEGs across cell types. g, log2FC values of the top DEGs for each cell type from MAST (top) and bulk RNA-seq (bottom). h, Overlap between n = 1,184 PTSD snDEGs and n = 1,918 MDD snDEGs. i, Top biological process (BP) and molecular function (MF) Enrichr Gene Ontology terms for the n = 502 PTSD-specific DEGs from panel h. j, Log-normalized mean expression of significantly discordant genes (CTNNA3 (top) and HSPA1A (bottom)) in each subtype for PTSD (blue) and MDD (orange). The asterisk indicates significance from rank–rank hypergeometric overlap (RRHO).
