Extended Data Fig. 3: Differential upregulation of the various splicing isoforms of CREM in NK cells.
From: CREM is a regulatory checkpoint of CAR and IL-15 signalling in NK cells
(a) Overview of the CREM gene. Created in BioRender. Rafei, H. (2025) https://BioRender.com/q33z837; (b) CREM expression in NT and CAR70/IL-15 NK cells using primers designed against CREM-specific exon, ICER-specific exon #1 or ICER-specific exon #2 as assessed by qPCR (n = 11 donors); (c) CREM expression in NK cells stimulated with increasing concentrations of IL-15 using primers designed against CREM-specific exon, ICER-specific exon #1 or ICER-specific exon #2 as assessed by qPCR (n = 10 donors); (d,e) whole cell lysates from CREM wild-type (WT) NT, CREM WT CAR70/IL-15 (d) or CAR.TROP2/IL-15 (e), and CAR-NK cells following CRISPR/Cas9 knockout (KO) using guides targeting the CREM-exon or ICER-exon or both as analyzed by western blot for CREM with β-actin serving as a loading control. ns: non-significant. Statistical comparisons were performed using t-tests (b) and two-way ANOVA with Tukey correction (c).
