Extended Data Fig. 6: Inhibition of KIR2DL1 activity by RIFINs.
From: RIFINs displayed on malaria-infected erythrocytes bind KIR2DL1 and KIR2DS1
a) NK expressing KIR2DL1 were obtained from a donor different from the one used in Fig. 3. Suppression of CD107a expression (left) and production of IFN-γ (center) and TNF-α (right) in these KIR2DL1-(+)-NK cells by RBK21, expressed on the surface of K562, is shown. K562 or K562 cells expressing ctl-RIFIN (PF3D7_1254200) were used as negative controls. Data represent the mean (n = 6 independent measurements from one donor), ****P < 0.0001 (two-sided Student’s t-test). b) The effects of RBK21 expressed on K562 on CD107a expression and production of IFN-γ production, and TNF-α in the KIR2DL1-(+) (top-left) and KIR2DS1-(+)- (bottom-left) donor-derived KIR2DL1/DS1-(–) NK cells are shown. Data represent the mean (n = 9 independent measurements from one donor). ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05 (two-sided Student’s t-test). c) RBK21 and PF3D71254200 were expressed as FLAG-tagged protein in K562 and their cell surface expression was confirmed using anti-FLAG antibody. d) Cytotoxic activities of NKL, NKL-KIR2DL1, and NKL-KIR2DL3 to K562 (left) and K562- expressing control RIFINs (PF3D7_1254200) (right) are shown. Data represent the mean with ± SD (n = 4 biological independent samples). The statistical analyses using two-sided Student’s t-test show that there are no significant differences between each sample. Exact P values for all panels in.
