Extended Data Fig. 1: Identification of KIR2DL1-binding iRBCs.
From: RIFINs displayed on malaria-infected erythrocytes bind KIR2DL1 and KIR2DS1
a) The integrity of each KIR-Fc protein was validated using conformational antibodies targeting each KIR37. Beads coated with KIR-Fc proteins are probed with anti-human-IgG and labelled-antibodies specific for KIR2DL1, DL2/DL3, DL5, 3DL1, and 3DL3. In the case of KIR3DL2, the assay was reversed such that the antibody was immobilized on the bead and probed with KIR3DL2-Fc, prior to secondary staining. b) The binding of 4 field-isolated parasites to each fluorescently labelled inhibitory KIR-Fc protein, assessed by flow sorting. The binding of Lek174 strain to KIR2DL1-Fc is shown in Fig. 1a. The positive iRBCs are highlighted in rectangles with the percentage of positive cells indicated.
