Extended Data Fig. 11: Analysis of Barnes Maze results.
From: Early life high fructose impairs microglial phagocytosis and neurodevelopment
(a, b) Evaluation of spatial learning and memory via the modified Barnes Maze. Experiments were performed as in Fig. 4 but used to assess spatial learning and memory. (a) Schematic of experiments used to analyze spatial learning and memory using the modified Barnes Maze. After three training days during which the escape hole was placed at the same location for three trials each day, the escape hole was then moved 180° on days 4 and 5 and two trials per day were performed. Created in BioRender; Saitz Rojas, W. (2025) https://BioRender.com/m9tkciq. (b) Each of the four cohorts were tested as outlined in (a), with the numbers of mice at N = 17 for WT CD, N = 16 for WT HF, N = 15 for KO CD, and N = 16 for KO HF. Data are shown as mean ± SEM. Significance was determined using two-way ANOVA with Tukey’s multiple comparisons test. ns = not significant.
