Extended Data Fig. 2: The effect of high fructose on neonatal microglia morphology in vivo.
From: Early life high fructose impairs microglial phagocytosis and neurodevelopment
(a) Schematics of microglial morphological features analyzed. Five features shown are highlighted in red. (b) High fructose diet does not affect branch number or length. Quantitation of branch number (4) and branch length (5) of microglia in the prefrontal cortex of P7 wildtype (WT) and Slc2a5–/– (KO) neonates born to dams on control diet (CD) or high fructose diet (HF) based on analysis of all cells from four fields of view (FOVs) per mouse obtained by confocal microscopy as in Fig. 1b. N = 24 for WT CD, N = 36 for WT HF, N = 32 for KO CD, and N = 20 for KO HF with n = 1056 to 1907 cells per condition. Each dot represents one cell whose morphological features were analyzed in a semi-automated process described in the Methods. Data are shown as mean ± SEM. Significance was determined by two-way ANOVA with Tukey’s multiple comparisons test. ns = not significant. Illustrations in a were created in BioRender; Saitz Rojas, W. (2025) https://BioRender.com/m9tkciq. (c) Maternal high fructose diet induces quiescent microglia morphology. Representative max intensity z-stack projections (left) of microglia in the prefrontal cortex from WT and KO P7 mice exposed to maternal CD or HF used to analyze microglia morphology. Quantitation of soma area (left graph), soma major axis length (middle), and soma roundness (right) from microglial morphological analysis of all cells from four FOVs per animal obtained by confocal microscopy as in Fig. 1b. N = 24 for WT CD, N = 36 for WT HF, N = 32 for KO CD, and N = 20 for KO HF with 1056 to 1907 cells per condition. Each dot represents one cell whose morphological features were analyzed in a semi-automated process described in the Methods. Data are shown as mean ± SEM. Significance was determined by two-way ANOVA with Tukey’s multiple comparisons test. ****p < .0001. Scale bar, 10 µm.
