Extended Data Fig. 8: Cryo-EM analysis of the SMARCAD1-nucleosome complex in Apo state.
From: Subnucleosome preference of human chromatin remodeller SMARCAD1
(a) A representative cryo-EM micrograph from 11,451 micrographs collected from one sample. Scale bar: 50 nm. (b) 2D class averages of characteristic projection views of cryo-EM particles. (c) Flowchart of the cryo-EM data processing. (d) Angular distributions of the cryo-EM particles in the final round of refinement. (e) Cryo-EM density map of the SMARCAD1-nucleosome complex coloured on the basis of the Local Resolution Estimation in cryosparc. The boxed regions are enlarged to show in (g). (f) Resolution estimation of the EM map. Gold standard Fourier shell correlation (FSC) curves, showing the overall nominal resolutions of 3.1 Å for the overall complex. (g) Local density map of the DNA around SHL2 of nucleosome. The structure of the canonical “601” nucleosome (PBD code 3MVD) was fit into map.
