Extended Data Fig. 5: Ku deletion in HCT116 also induces IFN signaling.
From: Ku limits RNA-induced innate immunity to allow Alu expansion in primates
a, PCR analyses show the specific and efficient deletion of the Ku86 flox allele upon 4OHT treatment. b, Progressive loss of viability in 4OHT treated Ku86Flox/−:CreERT2, but not control Ku86Flox/− cells. n = 6 from two independent experiments. Bars represent mean ± SD. The p-values were calculated via a two-way ANOVA test with Dunnett’s correction for multiple comparisons: p < 0.0001 ****; p > 0.05 n.s. c, Pathway analyses of significantly up-regulated genes upon Ku-deletion (96 hr). d and e, The GSEA for the interferon-pathway at 96 hr (4 days) after 4OHT treatment in control Ku86flox/− cells (d) or Ku86flox/−:CreERT2 (e). f, Heatmap of selective NF-kB targets in Ku-AID and Ku-flox cells. The color gradient reflects the Z-scores calculated from normalized TPM across the displayed samples: red represents higher expression levels relative to the mean, while blue indicates lower expression levels.
