Extended Data Fig. 9: Further characterization of uterine tumor cells and metastasis to lung.
From: Mouse lemur cell atlas informs primate genes, physiology and disease
a-c. H&E sections of primary endometrial tumor cells in the uterus of L2 (a, N = 2), which metastasized to the lung (b, close up of Fig. 3b, N = 1), and of endometrial tumor cells of L3 (c), which metastasized locally. Scale bar, 20 μm (all panels). Full set of micrographs are available on Tabula Microcebus web portal. d. UMAP of lung cells (left, L1-L4, 10x and SS2) and uterine cells (right, L3, 10x) integrated by FIRM and colored by (top to bottom) compartment, epithelial cell type designation (non-epithelial cells in grey), expression level of uterine marker OXTR, and expression level of human endometrial cancer marker MUC16/CA125, respectively. Note isolated cluster of lung epithelial cells (arrow, from L2), identified as metastatic tumor cells and cluster of uterine epithelial cells (arrowhead, from L3), designated as non-ciliated epithelial cell of uterus (FXYD4+ MUC16+), presumed to be primary endometrial tumor cells. e. UMAP of all atlas cell types showing their mean transcriptional similarity (L1-L4, 10x)1. Each data point is a cell type (unique combination of tissue and free annotations), colored by compartment. Boxed inset shows close-up of indicated UMAP region with cell types colored by compartment (circle fill) and tissue of origin (circle border), and labeled by cell type designation. Note metastatic tumor cells (Met, arrow) from L2 lung and adjacent (molecularly similar) uterine non-ciliated epithelial cells (FXYD4+ MUC16+, arrowhead) from L3, supporting uterine origin of the metastatic tumor.
