Fig. 1: Inhibition of R9AP impairs EBV infection in epithelial and B cells.
From: R9AP is a common receptor for EBV infection in epithelial cells and B cells
a,b, HNE1 cells were transfected with R9AP siRNA (siR9AP 1 or siR9AP 2) or control siRNA (siCtrl), then co-incubated with EBV. a, GFP expression was quantified by flow cytometry analysis and R9AP expression was analysed by western blot. b, Representative fluorescence microscopy images with EBV-positive cells shown in green. Scale bars, 100 μm. c,d, R9AP protein expression in CRISPR-edited HNE1 cells using control (sgVector) or independent R9AP sgRNA (sgR9AP 1 and sgR9AP 2) (c) and R9AP-reconstituted R9AP-knockout cells (d). EBV was added to the R9AP-knockout or reconstituted cells and EBV infection efficiency was analysed by flow cytometry. e,f, EBV infection in control and two R9AP-knockout single-cell HNE1 clones was measured by flow cytometry (f) and quantified (e, top). e, Bottom, R9AP protein expression in control cells and two R9AP-knockout HNE1 single-cell clones. g, R9AP protein expression in AGS cells expressing sgVector or R9AP sgRNA. EBV was added to AGS cells and infection efficiency was analysed by flow cytometry. h,i, EBV infection in control and two R9AP-knockout single-cell AGS clones was measured by flow cytometry (i) and quantified (h, top). h, Bottom, R9AP protein expression in control cells and two R9AP-knockout AGS single-cell clones. j, R9AP protein in control versus in R9AP-knockout Raji cells. EBV infection efficiency was analysed by flow cytometry. k,l, EBV infection in control and two R9AP-knockout single-cell Raji clones was measured by flow cytometry (l) and quantified (k, top). k, Bottom, R9AP protein expression in control cells and two R9AP-knockout Raji single-cell clones. Three independent experiments in triplicates (n = 9); data are mean ± s.e.m.; one-way ANOVA with Tukey’s correction for multiple comparisons (a,c–e,g,h,j,k). FSC, forward scatter.
