Extended Data Fig. 11: R9AP expression in human tonsil and blood tissues.
From: R9AP is a common receptor for EBV infection in epithelial cells and B cells
a, RT-qPCR was used to quantify the mRNA level of the R9AP in tonsil epithelial cells of 2 donors and memory B cells and naïve B cells from 2 donor’s blood and tonsil tissues. Data are mean ± S.E.M. (n = 3 biological replicates) and are from 2 donors. b, Blood PBMC were co-stained with CD19/R9AP antibody and analyzed by flow cytometry. Results are representative of 10 donors. c, Cells from tonsil tissues were co-stained with EpCAM/R9AP, CD19/CD27/R9AP, or CD19/CD10/R9AP antibody and analyzed by flow cytometry. Results are representative of 3 donors. d, e, Epithelium, and lymphoid tissues from tonsil were stained with hematoxylin-eosin staining (H&E), co-stained with R9AP (imaged as green) antibody and EpCAM (imaged as red) antibody, or co-stained with R9AP (imaged as green), CD19 (imaged as red) and CD27 (imaged as gray) antibodies, nuclei were stained with DAPI (blue), and analyzed by immunofluorescence. Epithelial cells were delineated by the dotted line (d). The white dotted areas indicated CD19+CD27+R9AP+ cells and were magnified 3 times, and the white solid areas indicated CD19+CD27−R9AP+ cells (e). Results are representative of 2 donors.
