Extended Data Fig. 4: R9AP mediates EBV entry and fusion.
From: R9AP is a common receptor for EBV infection in epithelial cells and B cells
a-d, Ectopic R9AP protein and EBV infection efficiency in CNE1 and AGS cells (a, b) or EBV-negative Akata cells (c, d). e, EBV binding in R9AP, CR2, or EV transiently transfected HEK-293T cells. f, g, EBV binding in R9AP or EV transiently transfected CNE1 cells or stably delivered EBV-negative Akata cells (f), in R9AP knockout HNE1 cells and EBV-negative Akata cells (g). h, i, EBV entry in R9AP or EV transiently transfected CNE1 cells or stably delivered EBV-negative Akata cells (h), in R9AP knockout HNE1 cells or EBV-negative Akata cells (i). j-l, Cell-based EBV fusion assay by co-culture of R9AP knockdown HEK-293T cells and HEK-293T cells transfected with gB and gH/gL (j), by co-culture of R9AP knockout Daudi cells, HEK-293T cells transfected with gB and gH/gL and His-gp42 protein at 0.5 μg/mL (k), by co-culture of R9AP overexpressed HEK-293T and HEK-293T cells transfected with gB, gH/gL, or gH/gL/gB (l). Three independent experiments in triplicates (n = 9) and mean and S.E.M. of those n = 9 were used (a, c, j, k, e), two independent experiments in triplicates (n = 6) and mean and S.E.M. of those n = 6 were used (f-i), or three independent experiments in quadruplicates (n = 12) and mean and S.E.M. of those n = 12 were used (l). Two-tailed unpaired Student’s t-test (a, c, f-k), one-way ANOVA was carried out with Tukey’s correction for multiple comparisons (e), or two-way ANOVA was carried out with Sidak’s correction for multiple comparisons (l). Data are representative of three independent experiments (b, d). NS, not significant.
