Extended Data Fig. 4: Cdc13-Cdc2 fusion protein mimics dynamics of uncomplexed Cdc13.
From: Spatiotemporal orchestration of mitosis by cyclin-dependent kinase
a) Single-cell traces of Cdc13-Cdc2-mNG mean intensities in nucleus (top) and cytoplasm (bottom). n = 89 cells; 1 of 3 biological repeats. b) Representative trace of Cdc13-Cdc2-mNG mean intensities in the nucleus and cytoplasm. c) Representative trace of NucCDK-mScarletI in Cdc13-Cdc2-mNG background. d) Representative trace of CytCDK V2-mScarletI in Cdc13-Cdc2-mNG background. e) Single-cell traces of Cdc13-Cdc2AF-mNG mean intensities in nucleus (top) and cytoplasm (bottom). n = 24 cells; 1 of 3 biological repeats. f) Representative trace of Cdc13-Cdc2AF-mNG mean intensities in the nucleus and cytoplasm. g) Single-cell traces of NucCDK sensor readout in Cdc13-Cdc2AF-mNG background. n = 24 cells; 1 of 3 biological repeats. h) Single cell traces of CytCDK V2 sensor readout in Cdc13-Cdc2AF-mNG background. n = 49 cells; 1 of 3 biological repeats. i) Min max normalized phase plots of the nucleus in the Cdc13-Cdc2AF-mNG background. n = 24 cells. j) Min max normalized phase plots of the cytoplasm in the Cdc13-Cdc2AF-mNG background. n = 49 cells.
