Extended Data Fig. 8: PAL-SA pathway is the primary SA biosynthetic route in rice disease response.
From: Complete biosynthesis of salicylic acid from phenylalanine in plants
a-c, The total SA (SA + SAG) (a), BB (b), and BS (c) contents in the leaves of the adult plants of WT (XS11 or ZH11) and osd1, osd2, osd3, and osd4 mutant plants at 48 hrs after Xoo inoculation; d, The total SA (SA + SAG) contents in the leaves of adult plants of WT (XS11 or ZH11) and osd1, osd2, osd3, and osd4 mutant treated with 200-μM CA, BB, BS or SA for 6 days; Data are means ± s.d.; n = 4 (a and d) and n = 3 (b and c) biologically independent samples. Statistical analysis was performed using one-way ANOVA of the LSD test, and the different lowercase letters represented the significant differences at the level of 0.05. e, Top images: Disease symptoms in the leaves of the WT (ZH11 and XS11), OSD-associated mutants, and the substrate- and product-fed mutants at 4 days post inoculation (dpi) by syringe-infiltrated Xoo. The brown spots indicate the cell death induced by hypersensitive response (HR), and the water-stained areas indicate susceptibility reaction. Bottom images: trypan blue staining of the leaves of the WT (ZH11 and XS11), OSD-associated mutants, and the substrate- and product-fed mutants at 3 dpi by syringe-infiltrated Xoo. The trypan blue-staining intensity of the infected leaves was used to visualize cell death levels in the leaves. Photos were captured by a stereo microscope. Scan bars, 2 mm. All experiments were repeated at least twice with similar results. SA, salicylic acid, SAG, SA-2-O-β-D-glucoside, BB, benzyl benzoate, BS, benzyl salicylate, CA, cinnamic acid.
