Extended Data Fig. 1: Characterization of the SA-deficient mutants of rice osd1, osd2, osd3, osd4, oskat1 kat2, tobacco nnd1, nnd3, and maize zsd3.
From: Complete biosynthesis of salicylic acid from phenylalanine in plants
a, Characterization of the allelic mutants of osd1. b, Quantification of SA and SAG contents in the leaves from adult plants of wild type (WT), osd1-2, osd1-3, osd1-4, osd1-5, and OSD1-C. FW, fresh weight. OSD1-C, the osd1-1 mutant complemented by OSD1. c-f, Characterization of osd2 (c), osd3 (d), osd4 (e) and oskat1 kat2 double mutants (f) generated by CRISPR/Cas9 technology. g, Quantification of SA and SAG contents in the leaves from the adult plants of ZH11 and oskat1 kat2 double mutant, Data are means ± s.d.; n = 3 (b) and n = 4 (g) biologically independent samples. Statistical analysis was performed using two-sided Student’s t-test. Exact P values are provided in the figure. All experiments were repeated at least twice with similar results. h-j, Characterization of nsd1 (h), nsd3 (i), and zsd3 (j) mutants generated by CRISPR/Cas9 technology. The mutation nucleotides are shown in red font. The target site and the rotospacer-adjacent motif (PAM) are shown in light and dark blue font respectively. The orange rectangles indicate the gene coding region and the intermediate lines indicate introns. Scale bars, 200 bp. SA, salicylic acid; SAG, SA-2-O-β-D-glucoside.
