Extended Data Fig. 10: Fibrotic-like assembloids resemble in vivo fibrosis models.
From: Mouse liver assembloids model periportal architecture and biliary fibrosis
a. Bulk RNA sequencing analysis of homeostasis and fibrotic-like assembloids after 7 days or 2.5 weeks of culture. Heatmap represents the log10(TPM + 1) (transcripts per million) values from the RNAseq for the indicated genes, Z-scored across each gene (n = 3 biological replicates). b. UMAP representation of fibrosis-like assembloids. c. UMAP representation of liver damage models and assembloid integration; green – Msc, magenta – cholangiocytes, blue – hepatocytes, cyan – liver progenitor-like cells (LPLC). d. UMAP representation of the liver damage model datasets (grey) compared to fibrotic-like assembloids (colour). e. GSEA of fibrosis-like and homeostasis from hepatocytes in assembloids (MSigDB_Hallmark_2020). NES, normalized enrichment score. f. GSEA of fibrosis-like and homeostasis from cholangiocytes in assembloids (MSigDB_Hallmark_2020). NES, normalized enrichment score. g. GSEA of fibrosis-like and homeostasis from mesenchyme in assembloids (MSigDB_Hallmark_2020). NES, normalized enrichment score. h. Correlation analysis of various damage models comparing liver tissue datasets with fibrosis-like assembloids (THIS STUDY).
