Extended Data Fig. 7: Differences in curli production correlate B. bacteriovorus defense patterns and low levels of curli production results in a turbid plaque phenotype.
From: Functional amyloid proteins confer defence against predatory bacteria
(a) Representative image of each ECOR strain plated on media containing Congo Red. Strains are grouped based on susceptibility and/or defense against B. bacteriovorus HD100 and 109J. (b-c) Efficiency of plating, calculated as PFU/mL (left axis), of B. bacteriovorus HD100 superimposed with the Congo Red score (right axis). The Congo red score was determined based on the about of red within a colony as shown in (a) (see Methods for more details). Data are the mean ± S.E.M. for n = 3 biological replicates. (b) Depicts ECOR1-48 and (c) depicts ECOR49-72. (d) Efficiency of plating, calculated as PFU/mL, of B. bacteriovorus 109J when infecting the indicated strains of E. coli. Data are graphed as in Fig. 1b. (e-f) Representative transmission electron microscopy images of the indicated strain of E. coli from three individual biological replicates. Cells expressing curli are indicated with a purple arrow and cells not expressing curli are indicated with a white arrow. Scale bars represent 500 nm. (g) Representative image of a plaque assay where the indicated strain of MG1655 was infected with HD100. Plaques are indicated with a black arrow. Data are representative of n = 3 biological replicates.
