Fig. 1: Divergence in structurally conserved regions corresponds to metabolic properties acting at the species, pathway and molecular levels.
From: The role of metabolism in shaping enzyme structures over 400 million years
a, Phylogenetic tree of the Saccharomycotina yeast subphylum highlighting the 26 species (+1 outgroup species) for which metabolic enzyme structures were generated and systematically compared. Colours indicate phylogenetic order, numbering counterclockwise starting at C. albicans. Branch lengths and topology are from the species time tree as calculated in ref. 2, except for the branch for the outgroup species S. pombe, which is not drawn to scale. b, Illustration of our analysis pipeline. c, Example alignment for 5-formyltetrahydrofolate cyclo-ligase structures in five species (S. cerevisiae, C. albicans, Kluyveromyces lactis, Kluyveromyces pastoris and S. pombe). The black line denotes the reference structure in S. cerevisiae (Fau1p). Insets show the orthologue from C. albicans with mapped residues (M, orange) and unmapped residues (N, cyan), as well as residues conserved (C, purple) between S. cerevisiae and C. albicans (purple). d, Mean mapping ratio (M/(M + N)) to mean conservation ratio (C/M) for the 529 reference structures that passed our filters. Dotted line denotes the identity line, the dashed line denotes the axis median and the solid line indicates the best linear fit. WGH, whole-genome hybridization.
