Fig. 2: Metabolic network organization constrains the structural evolution of enzymes in Saccharomycotina.
From: The role of metabolism in shaping enzyme structures over 400 million years
a,b, Mean conservation ratio per protein calculated for species that do or do not ferment glucose (a) and species that can or cannot grow on d-xylose (b), in refs. 17,18. For this analysis, orthogroups were temporarily subdivided on the basis of the phenotype of the species. For a, proteins with the largest differences in both directions, and, for b, pathways with remarkable changes, are highlighted. The dotted line denotes the identity line, the solid line denotes the linear fit and the dashed line denotes the axis median. c, Conservation ratio projected onto the yeast metabolic map of iPath3. d, Receiver operating characteristic (ROC) curve for four highly enriched pathways covering the TCA cycle and glucose metabolism. The number in the legend indicates the AUC. The dashed line denotes the identity line representing random sampling. e, Distribution of the mean conservation ratio of orthogroups assigned to oxidoreductases (red) or hydrolases (blue). The average distribution for all orthogroups is shown in black. f, Conservation ratio of enzymes known to bind metals (n = 158) and those not known to bind metals (n = 371), expressed as a box plot. The line denotes the median and the boxes denote the first and third quartile, the whiskers extend up to 1.5 times the IQR. Each dot represents an orthogroup. ***P < 1 × 10−4, two-sided Wilcoxon–Mann–Whitney U-test.
