Extended Data Fig. 4: The proteomics data are of high-quality. Metabolic properties influence structural evolution.
From: The role of metabolism in shaping enzyme structures over 400 million years
a) Completeness of proteomic measurements for the selected orthogroups. For 397 of the original orthogroups protein abundance was measured in at least one species. A black line indicates a measured protein abundance of the orthogroup in the respective species. The bar plots account for the row and column sum. Grey dotted lines indicate the first, second and third quartiles. b) Scatter plot of the technical coefficient of variation (quality control samples) versus the biological coefficient of variation (measured species). The dotted line denotes the identity line and the dashed line denotes the axis median. c) Median simulated flux compared to 13C experimental flux in 7 different datasets. The final plot compares median simulated flux to the median 13C experimental flux. Only reactions that had data from at least 5 studies were used. d) Spearman’s correlation of median predicted flux, coefficient of variations and Kendall’s τ correlation of the number of budding yeast species (n = 329) in which a flux>0 was predicted18 versus mean protein abundance and mean orthogroup conservation ratio. A * indicates an adj. p-value < 0.05. e) Mean conservation ratio versus standard deviation of the log2-transformed averaged kcat. Solid line is the best linear fit and the dashed line denotes the axis median.
