Extended Data Fig. 9: Pathogen growth in drug-altered Com20 is replicated in synthetic and stool-derived communities containing E. coli.
From: Non-antibiotics disrupt colonization resistance against enteropathogens
a) Classification of drug (columns) and concentration (rows) pairings according to the growth of S. Tm in drug-treated Com21. Samples marked with an X resulted in a community biomass <0.2 relative to an untreated community. b) Relative abundance of members of Com20 and Com21 in untreated controls (DMSO) and after treatment with diverse drugs. Abundances determined via 16S rRNA gene sequencing. Each bar represents one biological replicate. c) Biomass-scaled relative abundance of each member of drug-treated Com21, calculated by multiplying the normalized OD578 of the community by the relative abundance of each taxon obtained by 16S rRNA gene sequencing. Values indicate the mean of three biological replicates. d) IC25 values of 25 drugs in stool-derived communities of 8 healthy human donors. Tiles show the mean of three biological replicates. e) Association between the growth of S. Tm in Com20 (top) and Com21 (bottom), and the growth of the pathogen in stool-derived microbial communities across 10 drugs at various concentrations. Linear regression lines shown in red.
