Extended Data Fig. 2: Commensal gut bacteria are less resistant to non-antibiotic drugs than pathogenic Gammaproteobacteria species.
From: Non-antibiotics disrupt colonization resistance against enteropathogens
a) Inhibitory concentration of 25% growth inhibition (IC25) values for a panel of 19 gut commensals and 5 pathogenic taxa (Supplementary Table 2). The size of the circles indicate the percentage of species inhibited at a given concentration range. For example, clarithromycin inhibits 20% of pathogens at concentrations between 2.5 and 5 μM. For concentrations labeled as greater than (e.g. >20 μM), the inhibition threshold was not reached at the indicated value, and values greater than this (e.g. 40 μM) were not evaluated. Side heatmap shows the cumulative proportion of gut commensals and pathogens inhibited at a given concentration. b) and c) Chemical properties of compounds from the Prestwick library tested on pathogens (b) and commensals (c). Drugs were separated into 3 groups based on the number of bacteria they inhibited (Number of drugs in (b): No inhibition: 253, up to 3: 42, more than 3: 9. Number of drugs in (c): 0 to 1: 104, 2 to 5: 84, more than 5: 116). The properties assessed include molecular weight, hydrophobicity (XLogP), polar surface area (TPSA in Å2), complexity and 3D volume (in Å3). All properties were obtained from PubChem. Red horizontal and vertical lines represent mean ± 1 s.e.m. Adjusted P values < 0.05 from Kruskal-Wallis test followed by a Dunn’s test with Bonferroni correction are shown.
