Extended Data Fig. 5: Drug treatment can hamper the ability of Com20 to resist the growth of S. Tm and other Gammaproteobacteria pathogens.

a) Classification of drug (columns) and concentration (rows) pairings according to the growth of S. Tm in drug-treated Com20. Samples marked with an X resulted in a community biomass <0.2 relative to an untreated community and were excluded from downstream analyses. Note that zafirlukast tends to limit S. Tm growth, but due to high variance, it is not classified as S. Tm-restricting. b) Growth curves for pathogenic members of Gammaproteobacteria based on plating (top) or pathogen- specific luminescence (bottom). The curve indicates the mean of three biological replicates. The time point for luminescence measurements in the challenge assays was selected based on the plating results, indicated by vertical red lines. The growth of V. cholerae was not quantified by luminescence. c) Growth of Gammaproteobacteria species in untreated Com20 relative to pure culture. Pathogen loads from luminescence, except for V. cholerae, which was quantified by selective plating. Each point corresponds to a biological replicate. Red horizontal and vertical lines represent mean ± 1 s.e.m. of three biological replicates per condition d) Classification of drug-concentration pairings according to the growth of Gammaproteobacteria pathogens in Com20, similar to panel (a). Samples marked with an X resulted in a community biomass <0.2 relative to untreated Com20.

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