Extended Data Fig. 3: Validation of NINJ1-deficient animals, primary cells and THP-1 cells.
From: NINJ1 regulates plasma membrane fragility under mechanical strain
a, Mortality rate of the Ninj1−/− mice within 2 months from birth. b, Relative Ninj1 mRNA levels from the spleen of WT and Ninj1−/− mice. n = 4 animals per genotype. Two-sided unpaired Student’s t-test. * p < 0.05 versus WT. c, d, The LDH release and the percentage of DRAQ7+ primary BMDMs from Ninj1−/− mice and WT littermates after the application of 5 s stretch at 45% strain. Each data point is from 100 ~ 200 cells in one of the stretched wells. For each genotype, primary BMDMs were isolated and pooled from 4 animals. 2-way ANOVA followed by Bonferroni corrections. * p < 0.05, ** p < 0.01 vs WT. Unless otherwise noted, all data are presented as mean ± s.e.m. e, Sanger sequencing results of the NINJ1 KO THP-1 cells and the parental cells. The KO cells have 38 bp missing starting from base pair 202, causing a frameshift. f, Western blot of endogenous NINJ1 in the NINJ1 KO and the parental cells. GAPDH was used as the control for protein loading. Experiments have been repeated at least 3 times independently with similar results.
