Extended Data Fig. 12: External validation of DKD features.
From: Pathology-oriented multiplexing enables integrative disease mapping
(a) Cell types identified via single-cell RNA-sequencing. Even in early diabetic kidney disease, transcripts related to ER stress, Ca2+-mediated tubular injury, integrin beta 1-, and GR-signaling (b), as well as ECM remodelling and pro-fibrotic signaling (c) showed differential gene expression patterns compatible with differences observed at the protein level found in advanced diabetic kidney disease. SGLT2 inhibitor (SGLT2i)-treated patients exhibited incomplete modulation of transcriptomic changes. TAL: Thick ascending limb; PT: Proximal tubule; PC: Principal cell; ECs: Endothelial cells; IC: Intercalated cell; PC/CNT: Principal cells / Connecting tubule; DCT: Distal convoluted tubule; Mes/VSMC/Fib: Mesangial cells/Vascular smooth muscle cells/Fibroblasts; ATL/PEC: Ascending thin limb/Parietal epithelial cells; GECs: Glomerular endothelial cells; Mac/Mono: Macrophages/Monocytes; Lymph: Lymphocytes.
