Extended Data Fig. 4: Transcriptional profiling in experimental crescentic nephritis identifies JUN as a top regulated gene.

(a) Schematic description of experimental design with nephrotoxic serum (NTS) glomerulonephritis and glomerular nuclear isolation for bulk RNA sequencing. (b) Volcano plots show transcriptionally regulated genes at day 3 post NTS injection. (c) Transcription factor activity score, and (d) differential expression of JUN-regulated targets, where blue means downregulated by JUN and red means upregulated by JUN. Differential gene expression analysis was performed using PyDESeq2 combining single-factor analysis using Wald tests with log₂ fold-change shrinkage using approximate posterior estimation generalized linear models. Parts of panel a were created using BioRender (https://biorender.com).