Extended Data Fig. 7: Similarity of protein sequences recovered between CMNFV59632 and experimentally-heated samples.
From: Phylogenetically informative proteins from an Early Miocene rhinocerotid
Plots show sequence coverage for the three most abundant enamel matrix proteins (ENAM, AMELX & AMBN) for the Arctic Miocene rhino (CMNFV59632) tooth enamel and tooth enamel from Coelodonta antiquitatis that had been heated at 300 °C for 10 min, which showed similar levels of degradation (Extended Data Fig. 6). Peptides were retrieved from similar regions for ENAM and AMELX. The Cappellini et al. (2019) method2 was used to prepare Coelodonta antiquitatis enamel MS analysis, with some minor changes: HCl was used for demineralization instead of TFA, ZipTips (Millipore P10 with 0.6 μL C18 resin) were used instead of in-house StageTips and an Orbitrap Fusion Tribrid™ was used instead of a Q-Exactive for the proteomic analysis.
