Fig. 3: Abridged alignment and mirror plots of a phylogenetically informative SAP at AMELX-39.
From: Phylogenetically informative proteins from an Early Miocene rhinocerotid
Residue numbering (depicted above the alignment) follows the UniProt reference sequence F6QHS4 (F6QHS4_HORSE), corresponding to AMELX isoform 1 of Equus caballus. The upper spectrum is experimentally derived, whereas the lower one is predicted using the ‘Original mode’ with the Prosit tool, available online via the Universal Spectrum Explorer49. This spectrum is the highest scoring PSM (with Andromeda) for AMELX sequence positions spanning the most abundantly covered SAP differentiating between CMNFV59632 and all other rhinocerotids for which sequences are available. Instead, the more ancestral variant (YIDFSYEVLTPLK), shared with horses and others, is recovered.
