Extended Data Fig. 6: Impact of miR-2954 knockout on gene expression in heterozygous KO males.

Left column: log₂-fold changes (Log₂FC) in gene expression between Z^KOZ and ZZ genotypes for autosomal (n = 16,142) and Z-linked (n = 865) protein-coding genes; P-values from a two-sided Wilcoxon rank-sum test are indicated above. Log₂FC estimates are based on transcriptomes of E3 and E5 embryos across head, heart, and rest of the body tissues (n = 3 biological replicates per tissue, genotype, and developmental stage). Box plots show the median, 25th–75th percentiles, and whiskers extending to 1.5× the IQR. Right column: proportions of autosomal and Z-linked target and non-target genes among the differentially expressed (DE) genes (Benjamini-Hochberg adjusted P < 0.05) when comparing Z^KOZ and ZZ genotypes. Two-sided χ² test P-values are shown above.