Extended Data Fig. 10: Genes with miR-2954 target sites in birds and outgroup species.

a, Proportion of autosomal and Z-linked genes with predicted miR-2954 target sites (7–8mer matches) among all protein-coding genes in chicken, and among 1:1 chicken orthologs in zebra finch, ostrich, crocodile, and human. Distributions of predicted targets and non-targets were compared using two-sided χ² tests. The number of predicted targets and the total number of genes in each category are shown above the bars. b, Mean number of miR-2954 target sites per kilobase of 3′ UTR in chicken and human. A total of 11,789 autosomal and 571 Z-linked genes with annotated 3′ UTRs in both chicken and human were used to calculate the number of target sites per kilobase of 3′ UTR. Error bars indicate 95% confidence intervals from permutation tests (n = 10,000), with empirical P-values from two-sided pairwise permutation tests shown above the bars. In both panels, genes were classified as autosomal or Z-linked based on the chromosomal location of their 1:1 chicken orthologs.