Fig. 3: Expression patterns of miR-2954 and other miRNAs across tissues in control and KO embryos.

a, Distribution of expression levels (fragments per million mapped reads (FPM)) of miR-2954 in ZZ (n = 2), Z^KOZ (n = 3) and Z^KOZ^KO (n = 3) genotypes across head, heart and body at E5. Individual data points are overlaid with jitter. b, MA plot showing mean expression and log₂[FC] of mature miRNAs (n = 674) when comparing Z^KOZ^KO and ZZ genotypes across tissues. miRNAs with significant expression changes (Benjamini–Hochberg P_adj. value < 0.01) are shown in red. For miR-2954, Benjamini–Hochberg P_adj. values were less than 1 × 10⁻³⁸ for all tissues. c, Normalized expression (2^−ΔCT) of miR-2954 across the bursa of Fabricius (BF), bone, brain, heart, intestine, liver and muscle in male and female chicken embryos at E12 on the basis of reverse transcription–quantitative polymerase chain reaction (RT–qPCR) (n = 3). Benjamini–Hochberg P_adj. values from two-sided t-tests are shown above. Individual data points were overlaid with jitter. All box plots show the median, 25th–75th percentiles and whiskers extending to 1.5× the IQR.