Extended Data Fig. 7: Inhibition of the ImP/I1R axis with AGN192403 prevents ImP-induced immune and inflammatory response without affecting circulating ImP and cholesterol concentration.
From: Imidazole propionate is a driver and therapeutic target in atherosclerosis
a-i, ImP was administered (or not, CTR) in the presence of AGN192403 (AGN) or not (ImP) in drinking water to ApoE−/− mice fed chow diet for 8 weeks, followed by sacrifice and analysis. a, Oil red O en face staining of the aorta showing quantification of atherosclerotic lesions in whole aorta in male mice. CTR = 15; ImP=17; ImP + AGN = 16. b, Oil red O en face staining of the aorta showing quantification of atherosclerotic lesions in whole aorta (left), aortic arch (middle) and representative images (right) in female mice. CTR = 7; ImP=17; ImP + AGN = 16. c, Total cholesterol concentration in plasma. Male mice (left), CTR = 14; ImP= 13; ImP + AGN = 15, and female mice (right), CTR = 7; ImP=17; ImP + AGN = 14. d, Imidazole Propionate concentration in plasma of male mice at sacrifice. CTR = 10; ImP=13; ImP + AGN = 17. e,f, Number of T-bet in CD4+ T cells. (e), and CD90+ T and B220+ B cells (f) in blood at sacrifice by flow cytometry. CTR = 9; ImP=10; ImP + AGN = 10 (e) and n = 15 (f). g-j Number of aorta infiltrated T-bet+ Th1. CTR = 9; ImP=7; ImP + AGN = 7 (g) and FoxP3+ Treg cells CTR = 9; ImP=7; ImP + AGN = 7 (h), B220+ B cells. n = 10 (i) and F4/80+ Cd11c+ cells. CTR = 9; ImP=10; ImP + AGN = 10 (j) by flow cytometry. e,f,j, Right: representative density plots for CTR (top), ImP (middle) and ImP+AGN (bottom). a-j, Arithmetic mean ± SEM and individual data from at least two pooled independent experiments. One-way ANOVA with Tukey post-hoc correction.
